Therapeutic Effect of Arsenicum album on Leukocytes. Elaine C. Ive,1 Ingrid M. S. Couchman,1 and Lalini Reddy2* 1Department of Homoeopathy, Faculty of Health Sciences, Durban University of Technology, P.O. Box 1334, Durban 4000, South Africa; E-Mails: firstname.lastname@example.org (E.C.I.); Email: email@example.com (I.M.S.C.)2Department of Biotechnology and Food Technology, Faculty of Applied Sciences, Durban University of Technology, P.O. Box 1334, Durban 4000, South Africa. Int J Mol Sci. 2012; 13(3): 3979–3987. Published online 2012 March 22. doi: 10.3390/ijms13033979 PMCID: PMC3317753 Copyright © 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.
The therapeutic effects of homeopathic Arsenicum album potencies were investigated in-vitro, using a continuous cell line (MT4), pre-intoxicated with arsenic trioxide (As2O3), and then treated with succussed and unsuccussed homoeopathic potencies, 6CH, 30CH and 200CH.
This study aimed to verify the homoeopathic law of similars and to determine whether potencies diluted beyond Avogadro’s constant had physiological effects on cells; whether various potencies would cause different effects as suggested by the concept of hormesis; whether succussed and unsuccussed homeopathic potencies had different effects on the cells; and to establish whether a biotechnological method could be used to evaluate the above.
As2O3 was used to pre-intoxicate and the MTT assay was used to measure the percentage cytotoxicity and half maximal inhibitory concentration (IC50) of the cells. The homeopathic potencies of Arsenicum album (6CH, 30CH and 200CH) were prepared by either succussing or allowing to diffuse for 30 s.
After pre-intoxication of the MT4 cells with the IC50 As2O3 and treatment with succussed and unsuccussed Arsenicum album (6CH-200CH), the cell viability increased with increasing potency from 81% to 194% (over 72 h). The treatments and the times of exposure were found to be statistically significant determinants of cell viability, whereas succussion did not cause any significant variation in the results.
The study provided evidence that a biotechnological method (namely cell viability) may be used to scientifically evaluate the physiological effects of homeopathic potencies on human cells; it confirmed that the homeopathic potencies did have therapeutic effects; and that succussion was not required in the potentization method in order to produce a curative remedy.